Department of Bioproducts and Biosystems

Protein Technology

Professor Emma Master leads the Protein Technology research group. Our aim is to create breakthrough biotechnologies that customize nature’s most abundant structural biopolymers for use as building blocks in high-performance materials.
CHEM/BIO2/Protein technology group_enzyme research
Enzyme

Our approach applies functional genomics and biophysical methods to develop unique carbohydrate-active enzymes and non-catalytic proteins that control the chemical functionality and assembly of major lignocellulose components.

Research areas:

  1. Biocatalysts to upgrade renewable bioresources
  2. Protein Engineering
  3. Carbohydrate-active enzymes

By using biotechnologies to upgrade biopolymers for multipurpose and sustainable materials, we will:

  1. leverage environmental and economic benefits that are achieved when retaining naturally fixed CO2 in new bio-based products;
  2. establish biocatalysts as instruments for bio-based materials engineering;
  3. realize intact, structural biopolymers as the superior progenitor of high-performance materials.

Examples of ongoing research projects:

  • Carbohydrate-active oxidoreductases for bio-based cross linkers
  • Biocatalytic cascades for hemicellulose reassembly
  • Microbial expansin related proteins for bio-fibre engineering

Research aims to find tools for sustainable utilization of renewable plant resources

Forest biomass (lignocellulose) is largely made up of three polymers, cellulose, hemicelluloses, and lignin. Today, cellulose fibers are the main product of pulp mills across Finland and globally, while over 50% of the wood biomass resource remains underutilized. Our research focuses on the discovery and development of new proteins that can be used to sustainably synthesize novel, high-value biomaterials from underutilized and renewable plant resources.

Enzymes are tools with advantages

Enzyme catalyzed reactions benefit from four key advantages.

  1. Specificity that allows predictable modification of complex substrates.
  2. Exquisite tunability through protein discovery and engineering.
  3. Operation in mild reaction conditions that can reduce energy costs and undesired transformation of starting materials.
  4. Biodegradability, which helps to ensure the sustainability of the synthesis process as well as end product.

The Protein Technology group mines unexplored genomic data from plant biomass degrading microorganisms to discover new enzymes and non-catalytic proteins able to create valuable, bio-based products from plant polysaccharides. To support this objective, our group also designs and develops new enzyme screens to address the increasing limitations of existing assays.

Our research through images:

CHEM/BIO/Protein technology group_enzyme research 1

Enzyme research

CHEM/BIO/Protein technology group_enzyme research 2

Enzyme research

CHEM/BIO/Protein technology group_enzyme research overview

Enzyme research overview

CHEM/BIO/Protein technology group_bioinformatics research

Bioinformatics research

CHEM/BIO/Protein technology group_bioinformatics research

Bioinformatics research

CHEM/BIO2/Protein technology group_Time of Flight Secondary Mass Spectrometry

Time of Flight Secondary Mass Spectrometry (ToF SIMS) to image plant fiber after enzyme treatment

CHEM/Bio2/Protein technology group_plant fiber after enzyme treatment

Scanning Transmission X-ray Microscopy (STXM) to image plant fiber after enzyme treatment

CHEM/BIO/Protein Technology group_group picture

Related content:

Emma Master has received an ERC Consolidator Grant

The project aims to accelerate benefits of the genomic era, by finding novel proteins and enzymes with totally new and useful properties.

Portrait picture of Adjunct Professor Emma Master, Aalto University

Latest publications:

Discovery of fungal oligosaccharide-oxidising flavo-enzymes with previously unknown substrates, redox-activity profiles and interplay with LPMOs

Majid Haddad Momeni, Folmer Fredslund, Bastien Bissaro, Olanrewaju Raji, Thu V. Vuong, Sebastian Meier, Tine Sofie Nielsen, Vincent Lombard, Bruno Guigliarelli, Frédéric Biaso, Mireille Haon, Sacha Grisel, Bernard Henrissat, Ditte Hededam Welner, Emma R. Master, Jean Guy Berrin, Maher Abou Hachem 2021 Nature Communications

The coordinated action of glucuronoyl esterase and α-glucuronidase promotes the disassembly of lignin–carbohydrate complexes

Olanrewaju Raji, Jenny Arnling Bååth, Thu V. Vuong, Johan Larsbrink, Lisbeth Olsson, Emma R. Master 2021 FEBS Letters

Structural characterization of the family GH115 α-glucuronidase from Amphibacillus xylanus yields insight into its coordinated action with α-arabinofuranosidases

Ruoyu Yan, Weijun Wang, Thu V. Vuong, Yang Xiu, Tatiana Skarina, Rosa Di Leo, Paul Gatenholm, Guillermo Toriz, Maija Tenkanen, Peter J. Stogios, Emma R. Master 2021 NEW BIOTECHNOLOGY

Bromide-Based Ionic Liquid Treatment of Hardwood Organosolv Lignin Yielded a More Reactive Biobased Polyol

Maryam Arefmanesh, Thu V. Vuong, Justin K. Mobley, Mona Alinejad, Emma R. Master, Mojgan Nejad 2020 Industrial and Engineering Chemistry Research

Quantitative Comparison of Pyranose Dehydrogenase Action on Diverse Xylooligosaccharides

Johanna Karppi, Hongbo Zhao, Sun Li Chong, Antti E. Koistinen, Maija Tenkanen, Emma Master 2020 Frontiers in Chemistry

Enzymatic production of 4-O-methyl d-glucaric acid from hardwood xylan

Thu V. Vuong, Emma R. Master 2020 Biotechnology for Biofuels

Biocatalytic Production of Amino Carbohydrates through Oxidoreductase and Transaminase Cascades

Ville Aumala, Filip Mollerup, Edita Jurak, Fabian Blume, Johanna Karppi, Antti E. Koistinen, Eva Schuiten, Moritz Voß, Uwe Bornscheuer, Jan Deska, Emma R. Master 2019 ChemSusChem

A family AA5_2 carbohydrate oxidase from Penicillium rubens displays functional overlap across the AA5 family

Filip Mollerup, Ville Aumala, Kirsti Parikka, Yann Mathieu, Harry Brumer, Maija Tenkanen, Emma Master 2019 PloS one

Unravelling the role of secreted, non-hydrolytic fungal proteins in biomass modification

Mareike Monschein, A K M AI Amin Leamon, Eleni Ioannou, Zeynep Tacer Caba, Varis Jutta, Kirsi S. Mikkonen, Emma Master 2019
More information on our research in the Research database.
Research database
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