Public defence in Biotechnology, M.Sc. Zhu Ping

The title of the thesis: Expanding the toolbox for genetic manipulations in Methanosarcina acetivorans

Thesis defender: Zhu Ping
Opponent: Professor Bastian Molitor, University of Leipzig, Germany
Custos: Professor Silvan Scheller, Aalto University School of Chemical Engineering

Methanosarcina acetivorans is a model methane producer that can utilize many different one-carbon substrates. Genetic tools are needed to investigate its physiology and to extend the product scope beyond methane. The aim of the thesis was to investigate more genetic tools for M. acetivorans to facilitate the genomic manipulations such as genome editing, gene expression, and metabolic pathways engineering. As a result, the tools developed in this study facilitate precise genomic manipulations, which paves the way of exploring the physiological features and unlocking the biotechnological potentials of this organism.

Keywords: Genetic engineering, toolbox, CRISPR, promoter engineering, integration site, Methanosarcina acetivorans, Methanogen

Thesis available for public display 10 days prior to the defence at: https://aaltodoc.aalto.fi/doc_public/eonly/riiputus/

Doctoral theses in the School of Chemical Engineering: https://aaltodoc.aalto.fi/handle/123456789/51