Public defence in Biotechnolofy, M.Sc. (Tech.) Anni Nisov

Title of the thesis: Functionalisation strategies for plant proteins in meat analogues and solubility-dependent food applications

Doctoral researcher: M.Sc. (Tech.) Anni Nisov 
Opponent: Dr. Volker Lammers, DIL, Quackenbrük, Germany
Custos: professori Alexander Frey, Aalto University School of Chemical Engineering

Modifying plant proteins for better performance in food applications

Plant-based protein concentrates and isolates are developed for environmental and health reasons to reduce reliance on animal proteins. However, their performance in food applications is still limited compared to their animal counterparts. This dissertation aimed to tackle this challenge by investigating several functionalization strategies to improve plant protein performance in meat analogues and solubility-dependent food applications. The study investigated various plant protein isolates and concentrates, including rice, pea, wheat, oat, and rapeseed proteins.

The research demonstrated that chemical pH-shifting and enzymatic cross-linking and deamidation improved the fibrous structure formation ability of the studied rice, wheat, pea, oat, and rapeseed derived plant proteins that is a relevant indicator for meat-mimicking texture. Fermentation was successful in decreasing unwanted chemical-like off-flavours in rapeseed, but introduced bitterness, whereas chemical pH-shifting was found as a better alternative for targeted flavour modification. Additionally, limited enzymatic protein hydrolysis was found to enhance foaming and gelation properties of originally insoluble rice proteins.

The findings of the study can be applied to develop more appealing and functional plant-based meat analogues and ingredients, meeting the growing demand for sustainable and healthy plant based food products. This dissertation builds on existing research by providing scientific evidence but also practical strategies for food industry to improve plant protein functionality. The research also created tools to promote the use of fibre-rich plant protein concentrates over isolates, which can lead to improved sustainability and public health benefits through dietary changes.

 

→Thesis available for public display 10 days prior to the defence

Contact information:
M. Sc. (Tech.) Anni Nisov
[email protected]

→Doctoral thesis in the School of Chemical Engineering

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